PRECISION NICHE DESIGN - DROPLET MICROFLUIDICS

• Cho IS, Gupta P, Mostafazadeh N, Wong SW, Saichellappa S, Lenzini S, Peng Z, and Shin JW*. Deterministic single cell encapsulation in asymmetric microenvironments to direct cell polarity. Advanced Science 10: 2206014 (2023). [Link] (*Corresponding)
  
• Puttrich T, O'Donnell, Wong SW, Kotche M, Felder AE*, Shin JW*. Development of a programmable magnetic agitation device to maintain colloidal suspension of cells during microfluidic syringe pump perfusion. PLoS ONE 18: e0282563 (2023). [Link] (*Corresponding) News
  
• Wong SW, Lenzini S, Bargi R, Feng Z, Macaraniag C., Lee JC, Peng Z, and Shin JW*. Controlled deposition of three-dimensional matrices to direct single cell functions. Advanced Science 7: 2001066 (2020). [PDF] (*Corresponding) News
• Mao AS*, Shin JW*, Utech S, Wang H, Uzun O, Weitz DA, and Mooney DJ. Deterministic encapsulation of single cells in thin tunable microgels for niche modeling and therapeutic delivery. Nature Materials 16: 236-243 (2017) (*Co-first author).  [PDF]  News
  
• Kent RN 3rd, Said M, Busch ME, Poupard ER, Tsai A, Xia J, Matera DL, Wang WY, DePalma SJ, Hiraki HL, Killian ML, Abraham AC, Shin JW, Huang AH, Shikanov A, Baker BM. Physical and soluble cues enhance tendon protenitor cell invasion into injectable synthetic hydrogels. Adv. Funct. Mater. 32: 2207556 (2022).
• Wang WY, Kent RN 3rd, Huang SA, Jarman EH, Shikanov EH, Davidson CD, Hiraki HL, Lin D, Wall MA, Matera DL, Shin JW, Polacheck WJ, Shikanov A, Baker BM. Direct comparison of angiogenesis in natural and synthetic biomaterials reveals that matrix porosity regulates endothelial cell invasion speed and sprout diameter. Acta Biomater. 135: 260-273 (2021).
• Mao AS, Özkale B, Shah NJ, Vining KH, Descombes T, Zhang L, Tringides CM, Wong SW, Shin JW, Scadden DT, Weitz DA, Mooney DJ. Programmable microencapsulation for enhanced mesenchymal stem cell persistence and immunomodulation. Proc Natl Acad Sci U S A. 116: 15392-15397 (2019).

PRECISION NICHE DESIGN - PRINTING

• Devine D, Vijayakumar V, Wong SW, Lenzini S, Newman P, and Shin JW*. Hydrogel micropost arrays with single post tunability to study cell volume and mechanotransduction. Advanced Biosystems 2000012 (2020). [PDF] (*Corresponding)
• Newman PL, Yip Q, Osteil P, Anderson TA, Sun JQJ, Kempe D, Biro M, Shin JW, Tam PPL, Zreiqat H. Programming multicellular patterning with mechanochemically microstructured cell niches. Advanced Science 2204741 (2023). [Link]

ENGINEERING HOST REMODELING

• Wong SW, Tamatam C, Cho IS, Toth PT, Bargi R, Belvitch P, Lee JC, Rehman J, Reddy SR, and Shin JW*. Inhibition of aberrant tissue remodeling by mesenchymal stromal cells singly coated with soft gels presenting defined chemomechanical cues. Nature Biomedical Engineering (2022). [PDF] (*Corresponding) News & Views; News
• Wong SW, Lenzini S, Cooper MH, Mooney DJ*, and Shin JW*. Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and trafficking. Science Advances 6: eaaw0158 (2020). [PDF]  (*Corresponding)
• Singh A, Chakraborty S, Wong SW, Hefner NA, Stuart A, Qadir AS, Mukhopadhyay A, Bachmaier K, Shin JW, Rehman J, Malik AB. Nanoparticle targeting of de novo profibrotic macrophages mitigates lung fibrosis. Proc Natl Acad Sci U S A. 119: e2121098119 (2022).

EXTRACELLULAR NANOSCALE MEDIATORS IN CONTEXT

• Lenzini S, Debnath K, Joshi JC, Wong SW, Srivastava K, Geng X, Cho IK, Song A, Bargi R, Lee JC, Mo GC, Mehta D, and Shin JW*.  Cell-matrix interactions regulate functional extracellular vesicle secretion from mesenchymal stromal cells. ACS Nano (2021). [PDF]  (*Corresponding) News
• Lenzini S, Bargi R, Chung G, and Shin JW*.  Matrix mechanics and water permeation regulate extracellular vesicle transport. Nature Nanotechnology 15: 217-223 (2020). [PDF]  (*Corresponding) News & Views; Blog; News 

PHYSICAL APPROACHES TO BIOMATERIAL DESIGN

• Shin JW and Mooney DJ. Extracellular matrix mechanics causes systematic variations in proliferation and chemosensitivity in myeloid leukemias. Proceedings of the National Academy of Sciences (PNAS), USA 113: 12126-12131 (2016). [PDF]  News
• Mao AS, Shin JW, and Mooney DJ. Effects of substrate stiffness and cell-cell contact on mesenchymal stem cell differentiation. Biomaterials 98: 184-191 (2016).
• Chaudhuri O, Koshy S, Branco da Cunha CB, Shin JW, Verbeke CS, Allison KH, and Mooney DJ. Extracellular matrix stiffness and composition jointly regulate the induction of malignant phenotypes in mammary epithelium. Nature Materials 13: 970-8 (2014).

PHYSICAL CONTROL OF CELLULAR DIFFERENTIATION

• Shin JW, Buxboim A, Spinler KR, Swift J, Christian DA, Hunter CA, Leon C, Cachet C, Dingal PC, Ivanovska IL, Rehfeldt F, Chasis JA, and Discher DE. Contractile forces sustain and polarize hematopoiesis from stem and progenitor cells. Cell Stem Cell 14: 81-93 (2014) [PDF] (Cover Article).  Preview  News
• Shin JW, Spinler KL, Swift J, and Discher DE. Myosin-II inhibition and soft 2D matrix maximize multinucleation and cellular projections typical of platelet-producing megakaryocytes. Proceedings of the National Academy of Sciences (PNAS), USA 108: 11458-63 (2011). [PDF] News
• Buxboim A, Irianto J, Swift J, Athirasala A, Shin JW, Rehfeldt F, and Discher DE. Coordinated increase of nuclear tension and lamin-A with matrix stiffness outcompetes lamin-B receptor that favors soft tissue phenotypes. Mol Biol Cell 28: 3333-48 (2017).
• Spinler KR, Shin JW, Lambert M, and Discher DE. Myosin-II repression favors pre/pro-platelets, but shear-activation generates platelets and fails in macrothrombocytopenia. Blood 125: 525-33 (2015).
• Buxboim A, Swift J, Irianto J, Spinler KR, Dingal PC, Athirasala A, Kao YC, Cho S, Harada T, Shin JW, and Discher DE. Matrix elasticity regulates lamin-A,C phosphorylation and turnover with feedback to actomyosin. Current Biology 24: 1909-17 (2014).
• Swift J, Ivanovska IL, Buxboim A, Harada T, Dingal PC, Pinter J, Pajerowski JD, Spinler KR, Shin JW, Tewari M, Rehfeldt F, Speicher DW, and Discher DE. Nuclear lamin-A scales with tissue stiffness and enhances matrix-directed differentiation. Science 341: 1240104 (2013).
• Suzuki A, Shin JW, Wang Y, Lian L, Zhao L, Min SH, Discher DE, Poncz M, Choi JK, Carpenter C, and Abrams CS. RhoA is essential for maintaining normal megakaryocyte ploidy and platelet generation. PLoS ONE 8: e69315 (2013).

PHYSICAL CONTROL OF CELLULAR ADHESION AND MIGRATION

• Shin JW, Spinler KR, Swift J, Chasis JA, Mohandas N, and Discher DE. Lamins regulate cell trafficking and lineage maturation of adult human hematopoietic cells. Proceedings of the National Academy of Sciences (PNAS), USA 110: 18892-7 (2013). [PDF] Commentary
• Kruse K, Lee QS, Sun Y, Klomp J, Yang X, Huang F, Sun MY, Zhao S, Hong Z, Vogel SM, Shin JW, Leckband DE, Tai LM, Malik AB, Komarova YA. N-cadherin signaling via Trio assembles adherens junctions to restrict endothelial permeability. Journal of Cell Biology 218: 299-316 (2019).
• Harada T, Swift J, Irianto J, Shin JW, Spinler KR, Athirasala A, Diegmiller R, Dingal PC, Ivanovska IL, and Discher DE. Nuclear lamin stiffness is a barrier to 3D-migration, but softness can limit survival. Journal of Cell Biology 204: 669-82 (2014).
• Raab M, Swift J, Dingal PC, Shah P, Shin JW, and Discher DE. Crawling from soft to stiff matrix polarizes the cytoskeleton and phospho-regulates myosin-II heavy chain. Journal of Cell Biology 199: 669-83 (2012).

MULTISCALE DESIGN

• Khoo CK and Shin JW. Designing with biomaterials for responsive architecture. Material Studies. eCAADe Vol 2: 285-292 (2018). [PDF]
• Shin JW and Sabin JE. Tissue architecture: Programmable folding in digital responsive skins. Association for Computer-Aided Design in Architecture (ACADIA): Adaptive Architecture (2013). [PDF]

NEUROANATOMY & CHEMISTRY

• Shin JW, Nguyen KT, Pow DV, Knight T, Buljan V, Bennett MR, and Balcar VJ. Distribution of Glutamate Transporter GLAST in Membranes of Cultured Astrocytes in the Presence of Glutamate Transport Substrates and ATP. Neurochemical Research 34: 1758-66 (2009).
• Nguyen KT, Shin JW, Rae C, Nanitsos EK, Acosta GB, Pow DV, Buljan V, Bennett MR, Else PL, and Balcar VJ. Rottlerin Inhibits (Na+,K+)-ATPase Activity In Brain Tissue And Alters D-Aspartate Dependent Redistribution of Glutamate Transporter GLAST in Cultured Astrocytes. Neurochemical Research 34: 1767-74 (2009).
• Palombi O, Shin JW, Watson C, and Paxinos G. Neuroanatomical affiliation visualization-interface system. Neuroinformatics 4: 299-317 (2006).
• Nanitsos E, Acosta G, Saihara Y, Stanton D, Liao L, Shin JW, Rae C, and Balcar V. Effects of glutamate transport substrates and glutamate receptor ligands on the activity of Na-/K(+)-ATPase in brain tissue in vitro. Clinical and experimental pharmacology & physiology 31: 762-9 (2004).

Reviews and commentaries

• Gupta P, Alheib O, Shin JW*. Towards single cell encapsulation for precision biology and medicine. Advanced Drug Delivery Reviews 115010 (2023). [PDF]  (*Corresponding)
  
• Debnath K, Las Heras K, Rivera A, Lenzini S, Shin JW*. Extracellular vesicle-matrix interactions. Nature Reviews Materials 8: 390-402 (2023). [PDF]  (*Corresponding)
• Wong SW, Lenzini S, Giovanni R, Knowles K, Shin JW*. Matrix biophysical cues direct mesenchymal stromal cell functions in immunity. Acta Biomaterialia 133: 126-138 (2021). [PDF] (*Corresponding)
• Lenzini S, Devine D, Shin JW*. Leveraging Biomaterial Mechanics to Improve Pluripotent Stem Cell Applications for Tissue Engineering. Front Bioeng Biotechnol. 7: 260 (2019). [Link] (*Corresponding)
• Shin JW*. Squeezing cells through the epigenetic machinery. Proceedings of the National Academy of Sciences (PNAS), USA 115: 8472-8474 (2018). [PDF] (*Corresponding)
• Wong SW, Lenzini S, and Shin JW*. Biophysical regulation of cancerous and normal blood cell lineages in hematopoietic malignancies. APL Bioengineering 2: 031802 (2018). [PDF] (*Corresponding)
• Shin JW and Mooney DJ. Improving stem cell therapeutics with mechanobiology. Cell Stem Cell 18: 16-19 (2016). [PDF]
• Shin JW and Discher DE. Blood and immune cell engineering: cytoskeletal contractility and nuclear rheology impacts cell lineage and localization. BioEssays 37: 633-42 (2015). [PDF]
• Shin JW, Swift J, Ivanovska IL, Spinler KR, Buxboim A, and Discher DE. Mechanobiology of bone marrow stem cells: From myosin-II forces to compliance of matrix and nucleus in cell forms and fates. Differentiation 86: 77-86 (2013). [PDF]
• Ivanovska IL, Shin JW, and Discher DE. From matrix elasticity and contractile polarization to nuclear lamina remodeling in fates of marrow stem cells – a need for nano-physical characterizations. Trends in Cell Biology 25: 523-32 (2015).
• Ivanovska IL, Swift J, Irianto J, Spinler KR, Shin JW, Buxboim A, and Discher DE. Nuclear imaging in mechanobiology. Handbook of Imaging in Biological Mechanics. Chapter 32. CRC Press (2014).
• Swift J, Harada T, Buxboim A, Shin JW, Tang HY, Speicher DW, and Discher DE. Label-free spectrometry exploits dozens of detected peptides to quantify lamins in wildtype and knockdown cells. Nucleus 4: 450-459 (2014).
• Shin JW and Discher DE. Cell culture: Soft gels select tumor-initiating cells. News & Views. Nature Materials 11: 662-663 (2012).
• Raab M, Shin JW, Discher DE. Matrix elasticity in vitro controls muscle stem cell fate in vivo. Stem Cell Research & Therapy 1: 38 (2010).